Muscatine is a city in Muscatine County, Iowa, United States. The population was 22,886 in the 2010 census, an increase from 22,697 in the 2000 census. It is the county seat of Muscatine County. The name Muscatine is unusual in that it is not used by any other city in the United States. Muscatine is the principal city of the Muscatine Micropolitan Statistical Area (2000 census population 53,905), which includes all of Muscatine and Louisa counties.