Waynesburg is a borough in and the county seat of Greene County, Pennsylvania, United States, 60 miles (97 km) southwest of Pittsburgh. The population was 4,184 at the 2000 census. The region is underlaid with several strata of coking coal, including the Pittsburgh No. 8 seam, the Waynesburg seam and the Sewickley, or Mapletown seam. The area is also rich with coalbed methane, which is currently being developed from the several underlying coal seams as an alternative fuel source.